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MTHFR C677T assay-5919

Restricted to Allina Health and Affiliate provider orders only.

Test info

Test name:

MTHFR C677T assay

Test number:

5919

Excellian order number:

LAB5919

Abbreviation:

MTL

Alternate names:

Methylenetetrahydrofolate reductase

Specimen

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL

Volume:

3.0 mL

Collection instructions:

Only one (1) tube needs to be drawn for any combination of the following tests:
- Calreticulin Exon 9 Assay
- Factor V Leiden Mutation
- Factor 2 Mutation
- Hereditary Hemochromatosis
- JAK2 V617F Mutation Detection
- MTHFR C677T assay
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting

Transport container:

Lavender (EDTA), 4mL

Alternate specimen type:

ACD whole blood
Sodium citrate (Na cit) whole blood

Alternate collection container:

Lt blue Sodium citrate (Na Cit) - 2.7mL

Yellow ACD (A or B)

Alternate transport container:

Lt blue Sodium citrate (NaCit)

Yellow ACD (A or B)

Required forms/information:

Molecular Medicare billing request

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.

Complete and submit the form to notify us of the need for Allina Health Laboratory to bill insurance for Molecular testing performed
All information requested is required in order for your request to be completed

Transport and stability:

Ambient (preferred)

Refrigerated

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Improper blood/anticoagulant ratio
Delay in transport
Improper storage
Interfering substances

Performance

Performing lab:

AHL - Molecular Diagnostics: D

Days set up:

Varies

TAT:

8 days

Method:

Polymerase Chain Reaction (PCR) with RFLP analysis

Clinical and Interpretive info

Reference ranges:

Negative

Clinical information:

Due to lack of established clinical utility, MTHFR genotyping is no longer covered by most insurances. MTHFR genotyping should not be ordered as a part of the clinical evaluation for thrombophilia or recurrent pregnancy loss; fasting homocysteine is the preferred biomarker in these cases. The use of MTHFR genotyping should be restricted to symptomatic patients who have an increased basal homocysteine. The lack of evidence to support MTHFR variant testing is detailed in a 2013 practice guideline from the American College of Medical Genetics and Genomics (ACMG). MTHFR genotyping is considered investigational for nutritional, mental health or pharmacogenic assessment. The indication for testing must be provided at the time of order. This is a genetic test and needs to be performed only once in a lifetime

Billing

Preauthorization:

This test may require preauthorization from the insurance provider. Check the payer guidelines and, if needed, obtain the pre-authorization prior to sample collection.

CPT codes:

81291

Subject to medical necessity (Y/N):

Yes

Other billing information:

Medical necessity

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.

Complete and submit the form to notify us of the need for Allina Health Laboratory to bill insurance for Molecular testing performed
All information requested is required in order for your request to be completed

Molecular Medicare billing request

Tracking

Date created:

10/02/2003

Revised date:

07/27/2018

Review date:

01/15/2024