CD10 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

CD10 by IHC
12376 - Technical only, 12379 - Technical & interpretation
Common ALL antigen
  • All IHC stains will include a positive control tissue
  • CD10 is expressed on precursor B-lymphoblastic lymphomas, Burkitt's lymphomas, and on some precursor T-lymphoblastic leukemia/lymphomas
  • CD10 will also be expressed on many follicular lymphomas, some diffuse large B-cell lymphomas, and multiple myelomas
  • CD10 expression has been seen in hairy cell leukemia and chronic myelogenous leukemia
  • Endometrial stromal cells and their neoplasms (and decidua) are usually positive for CD10, which helps discriminate these neoplasms from smooth muscle tumors
  • Epithelium that stains for CD10 includes breast myoepithelial cells, bile canaliculi, fibroblasts, and brush border cells of kidney and gastrointestinal tract
  • Recent studies have shown that CD10 labels bile canaliculi in a similar pattern as polyclonal CEA; thus, this marker is helpful in identifying hepatocellular carcinomas from non-HCC
  • CD10 is also helpful in the diagnosis of renal cell carcinoma, and some pancreatic carcinomas, since these tumors are usually positive for this marker
  • Some melanomas have also been reported as positive for CD10



Submit a formalin-fixed, paraffin embedded tissue block


Formalin-fixed, paraffin embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair


AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info


If requested, an interpretive report will be provided



  • CD10 protein (zinc metallopeptidase) is found on the surface of a wide variety of hematopoietic and non-hematopoietic tissues
  • CD10 is a cell surface enzyme that reduces cellular response to peptide hormones by regulating local peptide concentration
  • CD10 is expressed in early lymphoid progenitors and normal germinal center cells

Staining pattern

  • Cell surface membrane staining in lymphoid cells
  • Cytoplasmic and /or membranous (including bile canalicular in HCC) in epithelial cells


  1. Kaufmann, O, et al: Immunohistochemical detection of CD10 with monoclonal antibody 56C6 on paraffin sections. Am J Clin Pathol 111; 117-122. 1999.
  2. Avery AK, et al: Use of antibodies to RCC and CD10 in the differential diagnosis of renal neoplasms. Am J Surg Pathol 24(2): 203-210, 2000.
  3. Chu PG, and Weiss LM et al: Utility of CD10 in distinguishing between endometrial stromal sarcoma and uterine smooth muscle tumors: an immunohistochemical comparison of 34 case. Mod Pathol, 14(5): 465-471, 2001.
  4. Notohara K, et al: Solid-pseudo-papillary tumor of the pancreas. Immunohistochemical localization of neuroendocrine markers and CD10. Am J Surg Pathol 24 (10):1361-1371, 2000.
  5. Borscheri N, et al: Canalicular immunostaining of Neprilysin (CD10) as a diagnostic marker for hepatocellular carcinomas. Am J Surg Pathol, 25(10): 1297-1303, 2001.


88342 - 1st stain
88341 - each additional stain