Alpha-fetoprotein by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

Alpha-fetoprotein by IHC
12376 - Technical only, 12379 - Technical & interpretation
  • All IHC stains will include a positive control tissue
  • Among hepatomas 10-70% have been reported as positive in most series. Among positive cases, the frequency of individual AFP-positive cells varies from 5-50% or more.
  • AFP positivity has also been reported in hepatoblastomas of childhood.
  • Primary cholangiocarcinoma is AFP negative.
  • Germ cell tumors:
  • Endodermal sinus tumors are usually positive for AFP.
  • Some embryonal carcinomas and teratomatous epithelium are also positive for AFP.
  • Seminomas/dysgerminomas, intratubular germ cell neoplasia and choriocarcinomas are negative for AFP.
  • Bladder tumors may occasionally produce AFP (especially if they are of urachal origin).

Tissue Localization of Alpha-Fetoprotein in Malignant Germ Cell Tumors

  Tumor type Percent positive
Testis Seminoma 0
  Teratoma 0  
  Embryonal carcinoma 47
  Endodermal sinus tumor 50
  Choriocarcinoma 0
Ovary Dysgerminoma 0
  Immature teratoma 0
  Embryonal carcinoma 70
  Endodermal sinus tumor 100  
  Choriocarcinoma 0



Submit a formalin-fixed, paraffin embedded tissue block


Formalin-fixed, paraffin embedded (FFPE) tissue block


Tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled
  • Insufficient tissue
  • Slides broken beyond repair


AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info


If requested, an interpretive report will be provided



  • Alpha-fetoprotein is a 64kD tumor-associated embryonal antigen synthesized during fetal life in the yolk sac, liver and gastrointestinal tract and produced by liver, yolk sac and some germ cell tumors of testicular and ovarian origin.

 Staining patterns

  • Cytoplasmic based staining


88342 - 1st stain
88341 - each additional stain