The following special sample handling procedure must be followed to avoid ex vivo uric acid degradation.
Samples must be centrifuged at 3,000 - 3,200 RPM for 5 minutes.
Samples are to be run immediately after centrifugation or the tube must be placed in an ice water bath and analyzed for uric acid within four (4) hours of collection.
Lt green plasma separator (PST)
Refrigerated/on ice - 4 hours
Rasburicase is a recombinant urate-oxidase enzyme approved for use by the FDA in patients who are at risk of developing tumor lysis syndrome or for the management of elevated uric acid levels. Rasburicase catalyzes enzymatic oxidation of uric acid into an inactive and soluble metabolite allantoin. At room temperature, ELITEK causes enzymatic degradation of the uric acid in blood, plasma, and serum samples potentially resulting in spuriously low plasma uric acid assay readings.