Determine abnormal T cell lymphoproliferative phenotype
Pathology orders only
Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting
Lavender (EDTA), 4mL
Body fluid
Bone marrow
Cerebrospinal fluid (CSF)
Tissue
Body fluid
Bone marrow
Bone marrow transport vial/tube
Cerebrospinal fluid (CSF)
Sterile vial/container
Tissue
Manual request clients: Complete the "Brief clinical information" and "What do you want answered by Flow?" areas on the request form. Include previous Allina case number, if applicable.
EDTA whole blood
Ambient
Body fluid
Refrigerated
Bone marrow
Ambient
Cerebrospinal fluid (CSF)
Refrigerated
Tissue
Refrigerated
Flow cytometry
Tubes and Markers:
Tubes:
T1, T2, T3*
* T3 tube reflexed for an additional charge, only if immature or lymphoblastic features are detected in the T1 and/or T2 tubes
Markers:
CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD16, CD19, CD45, CD56, CD57, HLA-DR, TCRαβ, TCRγδ, TRBC1
* If T3 tube is reflexed, additional markers include: CD1a, CD3(cytoplasmic), CD34, TdT(nuclear))