Pyruvic acid, whole blood-994

October 5, 2022 Due to manufacturer supply backorder, this test is unavailable

Test info

  
Pyruvic acid, whole blood
  
994
  
LAB994
  
MSO
  
Pyruvate
  

Increased pyruvic acid levels have been associated with diabetes mellitus, vitamin deficiencies, uremia, congestive heart failure, liver diseases, muscular dystrophy, thiamine deficiency, and neoplastic conditions.

Pyruvic acid is useful in assessing oxygen deprivation and provides an index of the severity of circulatory failure.

Specimen

  

Patient should be in a fasting and resting state (should not exercise).

  
Potassium oxalate/sodium fluoride whole blood
  

Grey Sodium flouride (NaFl)

Tube should be pre-chilled prior to sample collection

  
0.5 mL
  
0.2 mL
Submission of the minimum volume does not allow for repeat testing
  
  • Draw blood into chilled gray-top tube
  • Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting
  
  1. Immediately after blood is drawn, add 2 mL of whole blood from the gray-top tube to 2 mL of 8% perchloric acid (7 mL of 70% perchloric acid diluted to 100 mL with deionized water). Perchloric acid tube   
    1. Use 1:1 blood (ie, equal parts) to 8% perchloric ratio if less blood is obtained.
  2. Shake mixture vigorously for about 30 seconds.
  3. Refrigerate for five minutes to ensure complete protein precipitation.
  4. Centrifuge 5 to 10 minutes at approximately 1500xg.
  5. Immediately transfer the CLEAR SUPERNATANT to a Transfer vial/tube with cap - 12mL (LabCorp).
    1. A second centrifugation of the supernatant may be necessary to obtain a clear protein-free solution.
  6. Label the transport tube as "SUPERNATANT" and send to the laboratory.
  

Ambient (preferred) - 5 days

Refrigerated - 7 days

Frozen - 7 days

Freeze/thaw cycle - stable x3

  
  • Whole blood received
  • Grey-top tube not prepared with perchloric acid

Performance

  
LabCorp Burlington (004788): R-NX
  
Tu, Th
  
5 days
  

Pyruvate - lactate; spectrophotometry

Clinical and Interpretive info

  

0.3 - 0.7 mg/dL

Billing

  
84210
  
Result 2905-8

Tracking

  
05/23/2019
  
03/18/2022
  
03/17/2022