Detect resistance of HIV-1 to protease, reverse transcriptase, and integrase inhibitors

Lavender (EDTA), 4mL x 3

• Collect specimen in three Lavender (EDTA), 4mL tubes.
• Immediately following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting

1. Spin within six (6) hours of collection.
2. Transfer plasma to one or more Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp) tube(s), labelled as EDTA plasma
3. Immediately freeze specimen at - 20 °C. Do not place samples on dry ice until completely frozen.
4. Transport specimens frozen.

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

Frozen (strict) - 2 years

• Snap-cap or pop-top tubes should be avoided and may result in specimen rejection.
• PPT™ not centrifuged
• Insufficient volume
• Heparinized plasma
• Nonfrozen specimens
• Specimens exposed to repeated freeze/thaw cycles

Polymerase chain reaction (PCR) amplification and DNA sequencing