FUS by FISH-4280J

Test info

  
FUS by FISH
  
4280J
  
LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES
  
CG FUS
  
FUS
LGFMS
Low-grade fibromyxoid sarcoma
16p11
  

Rearrangement of the FUS gene (located at G-band region 16p11) is implicated in several chromosomal translocations associated with both solid tumors and leukemias.  A t(7;16)(q33;p11) involving the FUS gene is a characteristic feature of low grade fibromyxoid sarcoma and a t(12;16)(q13;p11) resulting in the fusion of the DDIT3 and FUS genes is present in more than 90% of myxoid and round cell liposarcomas.

Specimen

  
Tumor tissue
  

Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline.

  
5 mm (3)
  

Specimens should be immersed in sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one (1) hour of procedure

  
Tumor tissue FFPE block
Tumor tissue FFPE or slides
  
Block
Slides: 2 plus one H & E stained slide
  
  1. Fix the tissue in 10% formalin for 6 - 72 hours.
  2. Prepare a Formalin-fixed, paraffin-embedded (FFPE) tissue block

Either the entire block, or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing

  

FFPE tissue block or

Tissue section mounted on charged, unstained slides

  
  • Cytogenetics oncology requisition
  

Ambient

  
Slides - 6 months
Blocks will be returned post reporting
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper storage
  • Leaking container
  • Slides broken beyond repair

Performance

  
AHL - Cytogenetics: X
  
As needed
  
5 days
  

Fluorescence in situ hybridization (FISH)

Clinical and Interpretive info

  

Positive for rearrangement:  >2.5% of 200 nuclei

Negative for rearrangement: ≤2.5% of 200 nuclei

Billing

  
88377 – Quantitative Morphometric FISH

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred.

Tracking

  
03/11/2021
  
04/06/2021