Monitor residual AML blasts based on pre-treatment phenotype

Pathology orders only

Lavender (EDTA), 4mL

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

Lavender (EDTA), 4mL

Body fluid

• Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
• Do not add anticoagulant or holding media
• The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
• Include representative slides (will be returned)

Bone marrow

• Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
• Specimens collected in EDTA are acceptable, but not preferred
• Transfer to an anticoagulant free sterile tube
• If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable
• Submit representative slides (will be returned)

Cerebrospinal fluid (CSF)

• Collect 5 mL (minimum 1 mL) in a sterile vial/container.
• Do NOT add any anticoagulant or holding media
• The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
• Send representative slides (will be returned)

Tissue

• Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
• Send representative slides (will be returned)

Body fluid

Falcon vial/tube - 15mL

Bone marrow

Bone marrow transport vial/tube

Cerebrospinal fluid (CSF)

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

Manual request clients: Complete the "Brief clinical information" and "What do you want answered by Flow?" areas on the request form. Include previous Allina case number, if applicable.

EDTA whole blood

Ambient

Body fluid

Refrigerated

Bone marrow

Ambient

Spinal fluid

Refrigerated

Tissue

Refrigerated

• Improper label (unlabeled or mislabeled)
• Wrong container (anticoagulant or solution)
• Tissues submitted in saline or B5 fixative are not acceptable and will be rejected
• Improper blood/anticoagulant ratio
• Delay in transport
• Improper storage
• Inappropriate timing of collection
• Interfering substances
• Leaking container

Flow cytometry

Tubes and Markers:

Tubes: M1, M2, M4, M3*

*varies according to diagnostic phenotype

Markers: CD10, CD11b, CD13, CD14, CD15, CD16, CD19, CD33, CD34, CD38, CD45, CD45RA, CD56,  

CD64, CD65, CD117, CD123, CD300e, CD371, and HLA-DR