RUNX/RUNX1 or t(8;21) is a recurring abnormality in AML.  According to current NCCN AML guidelines, a t(8;21) result is classified as a favorable cytogenetics risk factor.

This test can be ordered individually or as part of the AML FISH Panel. See the panel test for more details

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

- or -

1. Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
2. Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting

Transfer aspirate from syringe to transport container. 

Submit entire specimen unspun.

Sterile vial/tube

or

Dk green Sodium heparin (Na hep), no gel

Bone marrow core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

Whole blood:

Dk green Sodium heparin (Na hep), no gel

Whole blood:

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting.

Submit entire specimen unspun

Bone marrow core:

Sterile vial/tube

Whole blood:

Dk green Sodium heparin (Na hep), no gel

AHL Cytogenetics Oncology Request

Ambient

• Improper label (unlabeled or mislabeled)
• Wrong container (anticoagulant or solution)
• Delay in transport
• Improper storage
• Leaking container
• Slides broken beyond repair

Fluorescence in situ hybridization (FISH)

Normal or Abnormal with interpretation

Medical necessity