Plasma cell heavy chains-12420

Test info

  
Plasma cell heavy chains
  
12420
  
LAB12420
  
Ig Heavy
  
Flow
Flow Cytometry
Immunophenotyping
Multiple Myeloma
MM
MGUS
Monoclonal gammopathy
Plasma cell
  
Tubes:
P1
P2
P3

Markers:
CD19
CD20
CD38
CD45
CD56
IgA (cytoplasmic
IgG (cytoplasmic)
IgM (cytoplasmic)
Kappa (cytoplasmic)
Lambda (cytoplasmic)
  

Determine the phenotype of abnormal plasma cells

Specimen

  
EDTA whole blood
  
  
4 mL
  
1.0 mL
  

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting

  

Lavender (EDTA), 4mL

  
Body fluid
Bone marrow
Spinal fluid (CSF)
Tissue
  
Varies; see Alternate collection instructions
  

Body fluid

  • Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
    • Do not add anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Include representative slides (will be returned)

Bone marrow

  • Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
    • Specimens collected in EDTA are acceptable, but not preferred
  • Transfer to an anticoagulant free sterile tube
  • If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable
  • Submit representative slides (will be returned)

Cerebrospinal fluid (CSF)

  • Collect 5 mL (minimum 1 mL) in a sterile vial/container.
    • Do NOT add any anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Send representative slides (will be returned)

Tissue

  • Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
  • Send representative slides (will be returned)
 
 
  

Body fluid:

Falcon vial/tube - 15mL

Bone marrow:

Bone marrow transport vial/tube

Cerebrospinal fluid (CSF)

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

  

Manual request clients: 

  • Complete the "Brief clinical information" and "What do you want answered by Flow?" areas on the request form.
  • Include previous Allina Health case number, if applicable.
  

EDTA whole blood:

Ambient

Body fluid:

Refrigerated

Bone marrow:

Ambient

Spinal fluid:

Refrigerated

Tissue:

Refrigerated

  
14 days
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
    • Tissues submitted in saline or B5 fixative are not acceptable and will be rejected
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Inappropriate timing of collection
  • Interfering substances
  • Leaking container

Performance

  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow cytometry

Clinical and Interpretive info

Billing

  
88184
88185 x 9

Tracking

  
08/04/2021
  
08/05/2021