Calreticulin exon 9 assay-12372

Test info

Calreticulin exon 9 assay

The Calreticulin Exon 9 assay detects insertions or deletions that result in frameshift mutations, which alter the resulting protein's function


EDTA whole blood
3.0 mL
  • Only one (1) tube needs to be drawn for any combination of the following tests:
    • Calreticulin exon 9 assay
    • Factor V leiden mutation
    • Factor 2 mutation
    • Hereditary hemochromatosis
    • JAK2 V617F mutation detection
    • MTHFR C677T
  • Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting

Lavender (EDTA), 4mL


Sodium citrate (Na cit) whole blood
ACD whole blood
Bone marrow

Whole blood:

Lt blue Sodium citrate (NaCit)

Yellow ACD (A or B)



Whole blood:

Lt blue Sodium citrate (NaCit)

Yellow ACD (A or B)

Bone marrow:

Lavender (EDTA), 4mL


Molecular Medicare billing request

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.

  • Complete and submit the form to notify us of the need for Allina Health Laboratory to bill insurance for Molecular testing performed
  • All information requested is required in order for your request to be completed

Ambient (preferred)

Refrigerated (acceptable)

  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Inappropriate timing of collection
  • Interfering substances
  • Leaking container
  • Slides broken beyond repair


AHL - Molecular Diagnostics: D
8 days

Polymerase Chain Reaction (PCR) + capillary electrophoresis

Clinical and Interpretive info


No mutation detected


These CALR mutations are seen in 67% to 88% of patients with essential thrombocythemia or primary myelofibrosis who lack JAK2 or MPL mutations (JAK2, MPL, and CALR mutations are mutually exclusive). CALR mutations are not limited to patients with myeloproliferative neoplasms as they can be seen infrequently in patients with myelodysplastic syndromes or mixed myelodysplastic/myeloproliferative syndromes. The Calreticulin Exon 9 Assay can be ordered as a reflex to a negative JAK2 V617F Mutation Detection. The reflex must be indicated on the request form or in the Excellian order. If ordered as a reflex, do not order the individual assay.


This test may require preauthorization from the insurance provider. Check the payer guidelines and, if needed, obtain the pre-authorization prior to sample collection.