Adenovirus DNA by rapid PCR-994

Test info

  
Adenovirus DNA by rapid PCR
  
994
  
LAB994
  
MSO
  

Specimens grossly contaminated with blood may inhibit the PCR and produce false-negative results. 

Specimen

  
Multiple; see Collection instructions
  

Submit only 1 of the following specimens:

Body fluid
0.5 mL of body fluid. Do not centrifuge. Send specimen refrigerated in a screw-capped, sterile vial. Maintain sterility and forward promptly. Specimens grossly contaminated with blood may inhibit the PCR and produce false-negative results. The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by adenovirus DNA is not likely.

Respiratory specimen
1 mL of bronchial washing, bronchoalveolar lavage, naso-pharyngeal aspirate or washing, sputum, or tracheal aspirate. Send specimen refrigerated in a screw-capped, sterile container. Maintain sterility and forward promptly.

Spinal fluid
0.5 mL of spinal fluid. Do not centrifuge. Send specimen refrigerated in a screw-capped, sterile vial. Maintain sterility and forward promptly. Specimens grossly contaminated with blood may inhibit the PCR and produce false-negative results. The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by adenovirus DNA is not likely.

Stool
1 g of fresh stool. Send specimen refrigerated in a plastic, screw-capped, sterile container. Maintain sterility and forward promptly.

Swab (Nasal, throat, respiratory, genital or eye/ocular)
Swab area with an Eswab culture transport swab; Place swab back into swab cylinder. If a culture transport swab is not available,

MicroTest M4-RT transport media vial/tube (T605)

is acceptable.

 

 

 

Send specimen refrigerated. Maintain sterility and forward promptly.

Tissue
Send fresh tissue refrigerated in a screw-capped, sterile container containing 1 mL to 2 mL of sterile saline or multi-microbe medium (M5) (Mayo supply T484). Maintain sterility and forward promptly.

Urine
1 mL from a random urine collection. Send specimen refrigerated in a screw-capped, sterile container. Maintain sterility and forward promptly.

  

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by adenovirus DNA is not likely. Maintain sterility and forward promptly. 

Body fluid
0.5 mL of body fluid. Do not centrifuge. Send specimen refrigerated in a screw-capped, sterile vial. 

Respiratory specimen
1 mL of bronchial washing, bronchoalveolar lavage, naso-pharyngeal aspirate or washing, sputum, or tracheal aspirate. Send specimen refrigerated in a screw-capped, sterile container. 


Spinal fluid
0.5 mL of spinal fluid. Do not centrifuge. Send specimen refrigerated in a screw-capped, sterile vial. 

Stool
1 g of fresh stool. Send specimen refrigerated in a plastic, screw-capped, sterile container. 

Swab
Place swab back into swab cylinder. If a culture transport swab is not available, M4 or M5 media is acceptable. Send specimen refrigerated. 

Tissue
Send fresh tissue refrigerated in a screw-capped, sterile container containing 1 - 2 mL of sterile saline or multi-microbe medium (M5) (Mayo supply T484). 

Urine
1 mL from a random urine collection. Send specimen refrigerated in a screw-capped, sterile container. 

  

Dependent on specimen type; see Collection and Processing instructions

  

Refrigerated (preferred) - 7 days

Frozen - 7 days

  
  • Calcium alginate-tipped swabs
  • Wooden-shafted swabs
  • Eswabs
  • Transport swabs containing gel
  • PCR testing cannot be added on to a sample that has been opened and/or used for other testing. If the sample has already been used for testing, a new specimen will need to be collected.

Performance

  
Mayo Clinic Laboratories (LADV): R-NX
  
Mo - Fr
  
2 - 5 days
  

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Clinical and Interpretive info

  

None detected

Billing

  
87798

Tracking

  
08/28/2008
  
11/30/2020
  
11/30/2020