CD25 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

CD25 by IHC
12376 - Technical only, 12379 - Technical & interpretation

All IHC stains will include a positive control tissue

  • CD25 identifies an antigen found in certain peripheral T-cell lymphomas (such as cutaneous t-cell lymphomas)
  • This marker can identify patients who are eligible to be treated with ONTAK (diftitoxin denileukin), which has shown significant success in the treatment of refractory peripheral T-cell lymphomas



Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair


AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info


If requested, an interpretive report will be provided



  • CD25 is expressed by peripheral T-cell lymphomas, and is useful in identifying patients who may be eligible for monoclonal directed therapy
  • CD25 has also been shown to identify neoplastic mast cells, and distinguish them from reactive mastocytosis
  • CD25 expression is seen in certain B cell malignancies, such as Hairy cell leukemia
  • Activated T and B cells can also express CD25

Staining pattern

  • Cell surface membrane


  1. Talpur R, et al: Treatment of refractory peripheral T-cell lymphoma with diftitoxin denileukin (ONTAK).
  2. Dang NH et al: Phase II study of denileukin diftitox for relapsed/refractory B non-Hodgkin's lymphoma. J Clin Oncol. 2004 Sep 7.
  3. Jones D et al: Degree of CD25 expression in T-cell lymphoma is dependent on tissue site: implications for targeted therapy. Clin Cancer REs. 2004 Aug 15; 10(16):5587-94.
  4. Sotlar K et al: CD25 indicates the neoplastic phenotype of mast cells; a noel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimens. Am J Surg Pathol 2004, 28:1319-1325.


88342 - 1st stain
88341 - each additional stain