All IHC stains will include a positive control tissue

• IHCstaining for the presence of pituitary hormones helps distinguish pituitary adenomas and the rare primary pituitary carcinomas from other tumors occurring in the region of the sella (such as craniopharyngioma, metastatic carcinoma, meningioma, lymphoma, leukemia and plasmacytoma), none of which contain or produce pituitary hormones
• Positive staining for specific pituitary hormones can help classify pituitary adenomas into subclasses based on the corresponding cell type present
• Negative staining for pituitary hormones does not rule out a pituitary adenoma since some contain none of the known adenohypophyseal hormones
• Gonadotrophic cell adenomas account for 10-15% of all pituitary adenomas. They usually stain for both LH and FSH

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• This antibody reacts with follicle stimulating hormone. It recognizes the gonadotrophic cells of the pituitary gland
• In the normal pituitary gland the gonadotrophs comprise about 10% of the cells and are scattered throughout the adenohypophysis with some concentration in the posterolateral region
• In some gonadotrophs, FSH and LH can be demonstrated in the same cell, but most gonadotrophs contain only one of the hormones

 Staining pattern

• Cytoplasmic based staining