Pemphigus/Pemphigoid profile I-994

Test info

  
Pemphigus/Pemphigoid profile I
  
994
  
LAB994
  
MSO
  
Cutaneous Immunofluorescence
  

The detection of anti-skin (IC and BMZ) antibodies aids in the diagnosis, and sometimes prognosis, of chronic vesicular-bullous diseases including pemphigus, pemphigoid, cicatricial pemphigoid, and epidermolysis bullosa acquisita (EBA).

Specimen

  
Serum
  
  
1.0 mL
  
0.5 mL
  

Immediately following collection, thoroughly mix sample by gently inverting 5 times

  
  1. Allow sample to clot for a minimum of 30 minutes
  2. Spin within two (2) hours of sample collection
  3. Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum
  4. Freeze
  
  
  1. Allow sample to clot
  2. Spin 
  3. Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum, within two (2) hours of sample collection
  4. Freeze
  

Frozen (preferred) - 1 year

Refrigerated - 5 days

Ambient - 48 hours

 

  
  • Grossly hemolysis
  • Lipemia
  • Icteric specimens

Performance

  
IMMCO Diagnostics Inc (122) via LabCorp (806399): R-NX
  
Mo - Fr
  
3 - 5 days
  

Semi-quantitative Indirect Immunofluorescence (IFA)

Substrate: Rhesus Monkey and Guinea Pig Esophagus/NaCl Split-Skin Substrate

Clinical and Interpretive info

  

An interpretive report will be provided

  

Epithelial intercellular antibodies are diagnostic for pemphigus and occur in over 90% of patients with active forms. Because of the species specificity of intercellular antibodies, the use of the dual substrates further distinguishes the intercellular antibodies of pemphigus vulgaris and vegetans from the antibodies of pemphigus foliaceus and erythematosus. Antibodies to basement membrane antigens of stratified squamous epithelium occur in about 70% of active bullous pemphigoid, 50% of vesicular pemphigoid and EBA and 10% of cicatricial pemphigoid patients.

Billing

  
86256 x 2

Tracking

  
05/24/2019
  
12/26/2019
  
05/20/2020