CD35 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

CD35 by IHC
12376 - Technical only, 12379 - Technical & interpretation
Anti-human dendritic reticulum cell

All IHC stains will include a positive control tissue

  • CD35 is useful in identifying the follicular dendritic cells in non-Hodgkin's lymphomas, seen as a sharply defined area of nodular staining
  • This antibody can also show a loose, ill-defined area of staining in some diffuse lymphomas
  • Precursor B NHL such as lymphoblastic lymphomas, and Burkitt lymphomas, plasmocytomas, and hairy cell leukemias show no staining for CD35
  • Nodular lymphocyte predominant Hodgkin's disease will show CD35 staining in a follicular pattern; all other types of HD are negative
  • CD 35 is most useful in a panel with CD21 and CD23 to identify follicular dendritic cell sarcoma/tumor



Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair


AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info


If requested, an interpretive report will be provided



  • CD35 antibody reacts with a receptor of the C3b fragment of compliment (To5)
  • CD35 labels follicular dendritic cells in lymphoid follicles, and to a lesser degree, mantle zone lymphocytes
  • CD35 stains the C3b receptor on epithelial cells of renal glomeruli
  • CD35 will also stain macrophages and histiocytes

Staining pattern

  • Membrane -based staining



  1. Chan JKC: Proliferative lesions of follicular dendritic cells: an overview, including a detailed account of follicular dendritic cell sarcoma, a neoplasm with many faces and uncommon etiologic associations. Adv Anat Pathol, 1997; 4(6); 347-411.
  2. Nguyen DT et al: Follicular dendritic cell sacrum. Identification by monoclonal antibodies in paraffin sections. Appl Immunohistochem 2:60-64.


88342 - 1st stain
88341 - each additional stain