Arginase-1 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

  
Arginase-1 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
  • All IHC stains will include a positive control tissue
  
  • Arginase-1 reactivity is seen in the vast majority of well-differentiated hepatocellular carcinomas (HCC), but may be absent in poorly differentiated HCCs
  • This antibody is more sensitive than HepPar-1 in identifying HCC2
  • This antibody is very useful with Glypican 3 in identifying scirrhous HCC (more useful than HepPar-1)
  • One study showed rare non-hepatic tumors with Arginase reactivity (cholangiocarcinoma and prostatic adenocarcinoma)2

References

  1. Krings G et al: Immunohistochemical pitfalls and the importance of glypican 3 and arginase in the diagnosis of scirrhous hepatocellular carcinoma. Modern Pathology (2013) 26, 782–791.
  2. Yan, BC et al: A New Immunohistochemical Marker of Hepatocytes and Hepatocellular Neoplasms. Am J Surg Pathol 2010;34:1147–1154).
  3. Ordonez NG: Arginase-1 is a novel immunohistochemical marker of hepatocellular differentiation. Adv Anat Pathol 2014;21:285–290.

Specimen

  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

Tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled
  • Insufficient tissue
  • Slides broken beyond repair

Performance

  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info

  

If requested, an interpretive report will be provided

  

Specifications

  • Arginase-1 is a urea cycle metalloenzyme that is expressed in liver
  • This antibody is a sensitive and specific marker that can be used to confirm hepatocellular differentiation and exclude other possible primaries

 Staining patterns

  • Strong diffuse cytoplasmic staining; patchy nuclear staining can also be seen

References

  1. Krings G et al: Immunohistochemical pitfalls and the importance of glypican 3 and arginase in the diagnosis of scirrhous hepatocellular carcinoma. Modern Pathology (2013) 26, 782–791.
  2. Yan, BC et al: A New Immunohistochemical Marker of Hepatocytes and Hepatocellular Neoplasms. Am J Surg Pathol 2010;34:1147–1154).
  3. Ordonez NG: Arginase-1 is a novel immunohistochemical marker of hepatocellular differentiation. Adv Anat Pathol 2014;21:285–290.

Billing

  
88342 - 1st stain
88341 - each additional stain

Tracking

  
05/01/2017
  
10/17/2018