Amyloid A by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

  
Amyloid A by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Serum Amyloid A
SSA
  
  • All IHC stains will include a positive control tissue
  
  • Most inflammatory HAs show strong SAA staining, which can be patchy or diffuse (>90%)
  • SAA is negative in most (83%) of FNH cases (rare cases show patchy or diffuse staining)

Specimen

  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

Tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled
  • Insufficient tissue
  • Slides broken beyond repair

Performance

  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info

  

If requested, an interpretive report will be provided

  

Specifications

  • This antibody labels amyloid A (AA) in tissues and can be used to classify AA-amyloidosis.
  • This antibody does not react with other amyloid types, such as Alzheimer’s amyloid plaques
  • SAA can be used in a panel with glutamine synthetase and beta catenin to help differentiate between hepatic adenomas (HA) and focal nodular hyperplasia (FNH)

Staining patterns

  • Strong cytoplasmic staining

 

References

  1. Linke RP: Monoclonal antibodies against amyloid fibril protein AA. Production, specificity and use for immunohistochemical localization and classification of AA-type amyloidosis. J Histochem Cytochem 1984;32:322-8.
  2. Shafizadeh N et al: Diagnosis of well-differentiated hepatocellular lesions: role of immunohistochemistry and other ancillary techniques. Adv Anat Pathol 2011;18:438-445).
  3. Joseph NM et al: Diagnostic utility and limitations of glutamine synthetase and serum amyloid-associated protein immunohistochemistry in the distinction of focal nodular hyperplasia and inflammatory hepatocellular adenoma. Modern Pathology (2014) 27, 62–72; doi:10.1038/modpathol.2013.114.

Billing

  
88342 - 1st stain
88341 - each additional stain

Tracking

  
03/02/2016
  
10/17/2018
  
10/02/2020