MAFB/IGH FISH-4280G (bone marrow) or 4280H (blood)

Test info

  
MAFB/IGH FISH
  
4280G (bone marrow) or 4280H (blood)
  
LAB4280G - CYTOGENETICS BONE MARROW STUDIES
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
  
MAFB
  
Myeloma
Myeloma FISH Panel
Plasma Cell Dyscrasia Panel
Plasma Cell Panel
FISH
Fluorescence In Situ Hybridization
MAFB/IGH
t(14;20)
  

Rearrangements of MAFB/IGH are recurring abnormalities in plasma cell dyscrasia disorders including Plasma Cell Myeloma.  According to current mSMART 3.0 myeloma guidelines, rearrangement of MAFB/IGH is classified as a high-risk cytogenetics category. 

This test can be ordered individually or as part of a Plasma Cell Dyscrasia FISH Panel (See the individual panel test for more details).

Specimen

  
Bone marrow aspirate
  

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

  

- or -

  1. Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
  2. Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting

  
5 mL
  
3 mL
  

Transfer aspirate from syringe to transport container.

  

Submit entire specimen unspun.  Specimen will be CD138 enriched for plasma cell isolation.

  

Sterile vial/tube

  

or

Dk green Sodium heparin (Na hep), no gel

  
Bone Marrow core
Sodium heparinized whole blood
Touch Prep slides
  

Bone Marrow Core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

or 

Whole blood:

Dk green Sodium heparin (Na hep), no gel

  
Bone Marrow core: 10 mm
Whole blood: 5 mL
  

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting.

 

  

Submit entire specimen unspun

  
  

AHL Cytogenetics Oncology Request

  

Ambient

  
Specimens retained until completion of all testing
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Delay in transport
  • Improper storage
  • Leaking container
  • Slides broken beyond repair

Performance

  
AHL - Cytogenetics: X
  
Mo - Fr
  
14 business days
  

Fluorescence in situ hybridization (FISH)

Clinical and Interpretive info

  

Normal or Abnormal with interpretation

Billing

  
88271x2
88275
88291
88299
  
Yes
  

Tracking

  
09/08/2022
  
09/08/2022