Bullous pemphigoid 180 and 230 antibody, IgG-14222

1/29/2024:  This test has been discontinued. To order a referred alternate, place an MSO order for LabCorp 163995 BP180 and BP230 IgG Antibodies

Test info

  
Bullous pemphigoid 180 and 230 antibody, IgG
  
14222
  
LAB14222
  
BP180&230
  
BP 180 and 230 IgG antibody
Bullous pemphigoid 180 and 230 antibodies

Specimen

  
Serum
  
  
2.0 mL
  
0.5 mL
  

Immediately following collection, thoroughly mix sample by gently inverting 5 times

  
  1. Allow sample to clot for a minimum of 30 minutes
  2. Spin 
  3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum, within 2 hours of sample collection
  
  
  1. Allow sample to clot
  2. Spin 
  3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum, within 2 hours of sample collection
  

Ambient (preferred) - 5 days

Refrigerated (OK) - 5 days

Frozen - 1 year

  
  • Grossly hemolyzed
  • Bacterial contamination
  • Lipemic specimen
  • Icteric specimen
  • Non-serum specimen types

Performance

  
Beutner Lab via LabCorp (831695): R-LC
  
7 days
  

Enzyme-linked Immunosorbent Assay (ELISA)

Clinical and Interpretive info

  

Negative

  

Bullous pemphigoid (BP) is an autoimmune mediated immunobullous skin disorder found mainly in the elderly population and is characterized by frequent occurring of tense blisters and erythema. Antibodies are directed to the basement membrane zone and are found in the serum of patients as linear IgG or C3 sediments. Target antigens of the autoantibodies in BP patient serum are BP230 and BP180, also called BPAG1 and BPAG2. Molecular weight of these antigens is 230 kD and 180 kD respectively. BP180 is thought to be the direct target of the autoantibody because of its location, and the autoantibodies against BP230 are thought to be secondarily produced. The antibodies against BP180 are thought to be pathogenic, because the rabbit antibody against mouse in the NC16a region of BP180 forms blisters similar to BP when injected into neonatal mice. The main epitope of BP180 is located in the region close to cell membrane called NC16a and most patient serum reacts with the recombinant NC16a protein. Serum levels of BP180 co-relate with the disease activity.

Billing

  
83516 x 2
  
Results 53842-1

Tracking

  
05/31/2019
  
07/11/2022
  
07/11/2022