Enterovirus, real-time PCR-994

Test info

  
Enterovirus, real-time PCR
  
994
  
LAB994
  
MSO
  
Enterovirus RT-PCR
Enterovirus Real-time PCR
  

An aid in the diagnosis of enteroviral infections.

Specimen

  
Nasopharyngeal (NP) swab
  
  

Nasopharyngeal (NP) swab collection:

  1. Tip the patient’s head back.
  2. Gently insert the NP swab into the nostril parallel to the palate (not upwards) until resistance is encountered or the distance is equivalent to that from the ear to the nostril of the patient, indicating contact with the nasopharynx.   
    1. If any resistance is met in the passageways, do not force the swab; back off and try reinserting it at a different angle, closer to the floor of the nasal canal, or try the other nostril.
  3. Gently rub and roll the swab for 10-15 seconds while the swab is in contact with the nasopharyngeal wall.
  4. The CDC recommends leaving the swab in place for several seconds to absorb secretions.
  5. Slowly remove the swab and place in the transport medium.
  6. Break the swab shaft so that it fits into the medium container and recap tightly.
  7. Label the specimen appropriately. Document the source “NP” on the label.

New England Journal of Medicine video on NP swab collection: Nasopharyngeal Swab Collection Video

 

  

Copan UTM-RT transport (purple cap)

 

  
Cerebrospinal fluid (CSF)
Stool/rectal swab
  
  
0.5 mL (minimum 0.2) CSF (noncentrifuged); 1 g fresh stool; or one rectal swab
  

CSF:

Sterile vial/container

Stool:

Screw cap plastic container (sterile)

  

Swab and Stool:

Ambient (preferred) - 7 days

Refrigerated - 7 days

Frozen - 7 days

CSF:

Ambient (preferred) - 7 days

Refrigerated - 7 days

Frozen - 90 days

  
  • PCR testing cannot be added on to a sample that has been opened and/or used for other testing. If the sample has already been used for testing, a new specimen will need to be collected.

Performance

  
LabCorp Burlington (138636): R-NX
  
6 days/week
  
4 days
  

Real-time reverse transcriptase polymerase chain reaction (RT-PCR)

Clinical and Interpretive info

  

Negative

Billing

  
87498
  
Result 29591-5

Tracking

  
05/30/2019
  
02/19/2020