Coxiella burnetii (Q fever), Molecular Detection, PCR, Tissue-994

Test info

  
Coxiella burnetii (Q fever), Molecular Detection, PCR, Tissue
  
994
  
LAB994
  

Aiding in the diagnosis of Coxiella burnetii infection (eg, Q fever) using tissue specimens

Specimen

  
Fresh tissue or biopsy (Lung, bone, liver, heart valve, aorta, or endocardium)
  

Sterile container

 

  
Entire collection or 5 mm (3)
  

Refrigerated (preferred) - 7 days

Frozen - 7 days

Ambient - No

  
1 week
  
  • Tissue in formalin, formaldehyde
  • Bone marrow
  • Decalcified bone
  • Bone marrow slides

Performance

  
Mayo Clinic Laboratories (CBRP): R-NX
  
Mo, We, Fr
  
3-7 days
  

PCR

Bacterial nucleic acid is extracted from the specimen using the automated MagNA Pure instrument. The purified DNA is placed on the LightCycler instrument, which amplifies and monitors by fluorescence the development of target nucleic sequences after each PCR cycle. A specific target sequence from Coxiella burnetii is amplified and the resulting segment is detected using specific hybridization probes. Detection of the C burnetii target is performed through melting curve analysis using the LightCycler software.

 

Clinical and Interpretive info

  

Negative

  

A positive result indicates the presence of Coxiella burnetii DNA.

 

A negative result indicates the absence of detectable C burnetii DNA, but does not negate the presence of the organism and may occur due to inhibition of PCR, sequence variability underlying primers or probes, or the presence of C burnetii DNA in quantities less than the limit of detection of the assay.

Billing

  
87797

Tracking

  
08/13/2022
  
09/14/2023
  
08/15/2022