CHOP by FISH-4280J

Test info

  
CHOP by FISH
  
4280J
  
LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES
  
CG CHOP
  
CHOP
DDIT3
Myxoid liposarcomas
12q13
  

Rearrangement of the DDIT3 (CHOP) gene (CHOP) located at 12q13 is the cytogenetic hallmark of myxoid liposarcomas.  A t(12;16)(q13;p11) resulting in the fusion of the DDIT3 and FUS genes is present in more than 90% of myxoid and round cell liposarcomas. A variant t(12;22)(q13;q12) resulting in the fusion of the DDIT3 and EWSR1 genes has also been reported.

Specimen

  
Malignant tissue (solid tumor or other)
  

Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

  
5 mm (3)
  

Place 5 mmtissue specimen in a sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one hour of procedure.

  

Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one hour of procedure.

  
Tumor tissue FFPE block
Tumor tissue FFPE slides
  

Formalin vial

  
  1. Fix the tissue in 10% neutral buffered formalin for 6 - 72 hours
  2. Prepare a formalin-fixed, paraffin-embedded (FFPE) tissue block

Either the entire block (an H&E will be cut and stained, and assessed by an HPA pathologist), or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing

  

Formalin-fixed paraffin-embedded (FFPE) tissue block or 5 micron cuts mounted on positively charged slides

  
  • Cytogenetics oncology request
  

Ambient

  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Delay in transport
  • Improper storage
  • Leaking container
  • Slides broken beyond repair

Performance

  
AHL - Cytogenetics: X
  
2 - 3 times per week
  
5 days
  

Fluorescence in situ hybridization (FISH)

Clinical and Interpretive info

  

Positive for rearrangement:  >3.0% of 200 nuclei

Negative for rearrangement: ≤3.0% of 200 nuclei

Billing

  
88377 – Quantitative Morphometric FISH

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred.

Tracking

  
03/11/2021
  
04/06/2021