Alpha-1-antitrypsin by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

Alpha-1-antitrypsin by IHC
12376 - Technical only, 12379 - Technical & interpretation
  • All IHC stains will include a positive control tissue
  • The primary use for this antibody is in identifying the accumulation of AAT granules in alpha-1-antitrypsin deficiency of the liver
  • AAT is a very useful marker in the work-up of alpha-1-antitrypsin deficiency, since although it is synthesized by the liver in this disease, it is not released into the circulation (thus, the serum is deficient)
  • This antibody is also useful in identifying tumors which can make this protein, such as liver neoplasms, endodermal sinus tumors, and for histiocytic related tumors
  • AAT is expressed in liver related proliferations as follows: focal nodular hyperplasia (56%), liver cell adenoma (68%) and hepatoma (89%)



Submit a formalin-fixed, paraffin embedded tissue block


Formalin-fixed, paraffin embedded (FFPE) tisue block


Tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled
  • Insufficient tissue
  • Slides broken beyond repair


AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info


If requested, an interpretive report will be provided



  • AAT is a glycoprotein that represents a major component of the alpha-1-globulin serum fraction (90%)
  • AAT is found in serum, as well as in other bodily fluids such as lymphatics, mucus, saliva, synovial fluid, GI secretions, semen, amniotic fluid and colostrum
  • The embryonal yolk sac is the initial site of AAT production
  • Although AAT is predominately made in the liver, it is also produced in histiocytes and monocytes

Staining patterns

  • Granular based cytoplasmic activity
  • In alpha-1-antitrypsin deficiency, the staining appears as large globules, particularly around the periphery of hepatocytes


  1. Palmer, P.E. et al; Am J Surg Pathol 2:275-281, 1978.


88342 - 1st stain
88341 - each additional stain