MDM2 by FISH-4280J

Test info

  
MDM2 by FISH
  
4280J
  
LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES
  
CG MDM2
  
Liposarcoma
MDM2
12q15
  

MDM2 gene amplification differentiates a pathologic diagnosis of well-differentiated liposarcomas from benign lipomas.

Specimen

  
Involved tissue (solid tumor or other)
  

Tissue specimens should be placed in a sterile cup with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one (1) hour of the procedure.

  

Paraffin block or 5 micron cuts mounted on positively charged slides

  
Formalin-fixed, paraffin-embedded (FFPE) tissue block or slides
  
  • Allow specimen to fix in 10% neutral buffered formalin for 6 - 72 hrs
  • Prepare a formalin-fixed, paraffin-embedded (FFPE) tissue block
  • Submit the FFPE tumor block (an H&E will be cut and stained, and assessed by an HPA pathologist) or 2 unstained slides and 1 H&E 
  

FFPE block

Slides

  
  • Cytogenetics oncology requisition
  • Document time in fixation – specimen should be fixed in 10% neutral buffered formalin for 6 - 72 hrs
  

Ambient

  
Slides - 6 months
Blocks will be returned post reporting
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper storage
  • Leaking container
  • Slides broken beyond repair

Performance

  
AHL - Cytogenetics: X
  
As needed
  
5 days
  

Fluorescence in situ hybridization (FISH)

Clinical and Interpretive info

  

Positive (Amplified) result – 25 cells scored with an MDM2:CEP12 ratio ≥2.0

Negative (Non-amplified) result - 25 cells scored with a MDM2:CEP12 ratio <2.0

Billing

  
88377 – Quantitative Morphometric FISH

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred

Tracking

  
03/11/2021
  
03/15/2021
  
04/06/2021