CD8 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test info

CD8 by IHC
12376 - Technical only, 12379 - Technical & interpretation

All IHC stains will include a positive control tissue

  • CD8 is used to identify cytotoxic/suppressor T cells
  • This antibody is used in a panel with other T cell markers, to assess antigen expression relationships of benign and malignant T cell populations; unexpected expression and or loss of CD8 can occur in neoplasms
  • CD4 is often used with CD8 to evaluate helper/suppressor relationships of T lymphocytes



Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair


AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination

Clinical and Interpretive info


If requested, an interpretive report will be provided



  • CD8 antibody reacts with the CD8 protein associated with the suppressor-cytotoxic subset of T lymphocytes
  • CD8 is expressed with CD4 in thymocytes and in a very small number of circulating lymphocytes

Staining pattern

  • Cell surface membrane staining


  1. Mason DY et al: Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence. J Clin Pathol 1992; 45:1084-8
  2. Macon WR, et al: T-cell subset analysis of peripheral T-cell lymphomas by paraffin section immunohistology and correlation of CD4/CD8 results with flow cytometry. Am J Clin Pathol, 109:610-617, 1998


88342 - 1st stain
88341 - each additional stain