Lambda light chains by in situ hybridization

Alphabetical Test listing

Lambda light chains by in situ hybridization-12376 - Technical only, 12379 - Technical & interpretation

  
Lambda light chains by in situ hybridization
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Lambda
Lambda ISH
  
  • All ISH stains will include a positive control tissue
  

Kappa and Lambda stains are used to determine monoclonality in malignant plasmacytic or lymphoid populations, and polyclonality in benign populations

  
Tissue
  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

In-situ hybridization(ISH) and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

Lambda antibody reacts with free kappa chains and kappa chains in immunoglobulin complexes

Staining pattern

  • Cytoplasmic staining is present in plasma cells, and in plasmacytoid lymphocytes
  • Cell surface membrane staining can be seen on B-lymphocytes (this pattern is less likely to be identified on paraffin sections, but is more easily identified on frozen tissue)
  • Since endogenous immunoglobulin is present in all human tissues, diffuse "background" staining is expected.

References

1. Wolf BC, et al: An evaluation of immunohistologic stains for immunoglobulin light chains in bone marrow biopsies in benign and malignant plasma cell proliferations. Am J Clin Pathol 94:642-746, 1990.

  
88365 - 1st stain
88364 - each additional stain
  
07/17/2017
  
10/19/2018