Lambda light chains in situ hybridization (ISH)
Lambda light chains in situ hybridization (ISH)-12376 - Technical only, 12379 - Technical & interpretation
LAB12379
Lambda ISH
- All ISH stains will include a positive control tissue
Kappa and Lambda stains are used to determine monoclonality in malignant plasmacytic or lymphoid populations, and polyclonality in benign populations
Formalin-fixed, paraffin embedded (FFPE) tissue block
Submit a formalin-fixed, paraffin embedded tissue block
Formalin-fixed, paraffin embedded (FFPE) tissue block
FFPE tissue section mounted on a charged, unstained slide
Ambient (preferred)
- Unlabeled/mislabeled block
- Insufficient tissue
- Slides broken beyond repair
In-situ hybridization(ISH) and microscopic examination
If requested, an interpretive report will be provided
Specifications
Lambda antibody reacts with free kappa chains and kappa chains in immunoglobulin complexes
Staining pattern
- Cytoplasmic staining is present in plasma cells, and in plasmacytoid lymphocytes
- Cell surface membrane staining can be seen on B-lymphocytes (this pattern is less likely to be identified on paraffin sections, but is more easily identified on frozen tissue)
- Since endogenous immunoglobulin is present in all human tissues, diffuse "background" staining is expected.
References
1. Wolf BC, et al: An evaluation of immunohistologic stains for immunoglobulin light chains in bone marrow biopsies in benign and malignant plasma cell proliferations. Am J Clin Pathol 94:642-746, 1990.
88364 - each additional stain