CLL full panel by FISH

Alphabetical Test listing

CLL full panel by FISH-Order the individual components; 4280H (blood) or 4280G (bone marrow)

  
CLL full panel by FISH
  
Order the individual components; 4280H (blood) or 4280G (bone marrow)
  
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
LAB4280G - CYTOGENETIC BONE MARROW STUDIES
  
ATM deletion
CCND1/IGH
CLL
Chronic Lymphocytic Leukemia
Deletion 6q21
MYC rearrangement
SLL
Small Lymphocytic lymphoma
t(11;14)
Trisomy 12
TP53 deletion
8q24
11p22 deletion
+12
13q14.3 deletion
13q deletion
17p13.1 deletion
  
  • 6q21
  • ATM
  • CCND1
  • CEP12
  • DEL13
  • IGH
  • MYC
  • TP53

 

 

  

Detecting the prognostically significant cytogenetics FISH abnormalities used in the CLL/SLL NCCN guidelines

  
Sodium heparin (Na hep) whole blood
  
  
5 mL
  
2.0 mL
  

Only one specimen tube is required to perform all ordered cytogenetics testing.

The more testing ordered, the closer to the optimal volume requirements is preferred.

  

Dk green Sodium heparin (Na hep), no gel

  
Bone marrow
EDTA whole blood (a chromosome analysis study is not recommended for this specimen type)
  

EDTA whole blood (not recommended)

Lavender (EDTA), 4mL

  
Bone marrow: 10 mL (minimum 5 mL)
  

Bone marrow

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

  

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel

  

Bone marrow:

Sterile vial/tube

Dk green Sodium heparin (Na hep), no gel

EDTA whole blood:

Lavender (EDTA), 4mL

  
  • AHL Cytogenetics oncology request
  

Ambient

  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Leaking container
  
AHL - Cytogenetics: X
  
As needed
  
5 - 7 days
  

Fluorescence in situ hybridization (FISH)

  

Normal or Abnormal with interpretation

  

Deletion of ATM or TP53 are classified as unfavorable cytogenetics prognostic indicators in CLL/SLL.

Trisomy 12 and deletion of 13q14.3 are classified as neutral, however, CLL cases with a high proportion of cells with a deletion 13q14.3 do not do well.

6q21 deletions do not have any particular prognostic significance but help identify CLL patients when other FISH is negative.

MYC rearrangement is an indication of potential histologic (Richter’s) transformation to a more aggressive lymphoma.

CCND1/IGH is strictly used on newly diagnosed CLL/SLL patients in order to rule out mantle cell lymphoma.

  
88271 x 8
88275 x 4
  
Yes
  
03/18/2021
  
04/06/2021