ATM/TP53 by FISH
ATM/TP53 by FISH-4280H (blood) or 4280G (bone marrow)
LAB4280G - CYTOGENETIC BONE MARROW STUDIES
CLL Full Panel
CLL Prognostic Panel
Chronic Lymphocytic Leukemia
SLL
Small Lymphocytic lymphoma
TP53
TP53 Deletion
11q-
11q22
17p13.1
Deletion of 11q22 (ATM) and 17p13.1 (TP53) are recurring abnormalities of Chronic Lymphocytic Leukemia (CLL)/ Small Lymphocytic lymphoma (SLL).
This test can be ordered individually or as part of a CLL Full Panel or CLL Prognostic Panel (see the individual panel tests for more details).
- Only one specimen tube is required to perform all ordered cytogenetics testing.
- The more testing ordered, the closer to the optimal volume requirements is preferred.
- Immediately following collection, mix sample by inverting 8 - 10 times to prevent clotting
Dk green Sodium heparin (Na hep), no gel
EDTA whole blood (a chromosome analysis study is not recommended for this specimen type)
Bone marrow
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
EDTA whole blood:
Lavender (EDTA), 4mL
Bone marrow:
Sterile vial/tube
Dk green Sodium heparin (Na hep), no gel
- AHL Cytogenetics oncology request
Ambient
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Improper blood/anticoagulant ratio
- Delay in transport
- Improper storage
- Leaking container
Fluorescence in situ hybridization (FISH)
Normal or Abnormal with interpretation.
A deletion of either 11q22 (ATM) or 17p13.1 (TP53) is classified as an unfavorable cytogenetics prognostic indicator in CLL/SLL according to NCCN.