NKX3.1-PR by IHC

Alphabetical Test listing

NKX3.1-PR by IHC-12376 - Technical only, 12379 - Technical & Interpretation

  
NKX3.1-PR by IHC
  
12376 - Technical only, 12379 - Technical & Interpretation
  
LAB12376
LAB12379
  
  • All IHC stains will include a positive control tissue
  
  • NKX3.1 can be used to identify prostatic adenocarcinoma, in particular when metastatic, with a higher sensitivity and specificity than either PSA or PSAP
  • NKX3.1 will also stain nodular hyperplasia of the prostate
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • NKX3.1 is an androgen-regulated homeodomain gene whose expression is predominantly localized to prostate epithelium
  • NKX3.1 is located on chromosome 8p21.2, a region that shows loss of heterozygosity (LOH) in 12-89% of high-grade prostatic intraepithelial neoplasia (PIN) and 35% to 86% of prostatic adenocarcinomas
  • Expression of NKX3.1 is highly specific to prostate cancer but has been also seen in breast cancer (invasive lobular carcinoma), with little or no staining in a large number of other tumor types

Staining pattern

  • Strong nuclear staining
  • Negative staining includes cytoplasmic (non-nuclear) staining patterns

References

  1. Bethel CR, Faith D, Li X, Guan B, Hicks JL, Lan F, Jenkins RB, Bieberich CJ,De Marzo AM. Decreased NKX3.1 protein expression in focal prostatic atrophy, prostatic intraepithelial neoplasia, and adenocarcinoma: association with gleason score and chromosome 8p deletion. Cancer Res. 2006 Nov 15;66(22):10683-90. PubMed PMID: 17108105.
  2. Gurel B, Ali TZ, Montgomery EA, Begum S, Hicks J, Goggins M, Eberhart CG,Clark DP, Bieberich CJ, Epstein JI, De Marzo AM. NKX3.1 as a marker of prostatic origin in metastatic tumors. Am J Surg Pathol. 2010 Aug;34(8):1097-105. doi:10.1097/PAS.0b013e3181e6cbf3. PubMed PMID: 20588175; PubMed Central PMCID: PMC3072223.

 

  
88342 - 1st stain
88341 - each additional stain
  
07/18/2017
  
06/25/2019