Smooth muscle myosin heavy chain 1 by IHC

Alphabetical Test listing

Smooth muscle myosin heavy chain 1 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
Smooth muscle myosin heavy chain 1 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
SMMS
  
  • All IHC stains will include a positive control tissue
  
  • SMMSHC-1 will stain myoepithelial cells of ducts and acini in normal breast lobules. It will also react with vascular smooth muscle
  • SMMSHC-1 reacts with the intact myoepithelial cell layer present in benign and in situ breast lesions. It is useful in distinguishing benign breast and DCIS from invasive tumors of the breast
  • Rarely, reactivity is seen with myofibroblasts in desmoplastic stroma of invasive carcinoma (5 of 70 cases). When positive, only rare stromal cells were immunostained. 1
  • Myoepithelial layers in complex sclerosing lesions and papillomas stain positively along papillary fronds with SMMSHC-1 but no myoepithelial immunostaining along the papillary fronds has been observed in intracystic papillary carcinoma
  • SMMSHC-1 has been reported to label neoplastic myoepithelium of pleomorphic adenomas of the salivary gland 2
  • It is not yet known how SMMSHC-1 reacts in micro-glandular adenosis. Micro-glandular adenosis is a benign lesion of the breast composed of a single layer of epithelial cells, which has been reported to be devoid of a surrounding myoepithelial layer (i.e. MSA negative).
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • Antibody recognizes a cytoplasmic protein (SM-MHC, smooth muscle myosin heavy chain) which is a major component of the contractile apparatus in smooth muscle cells
  • 2 isoforms of SM-MHC exist: SM1 (204 kd) and SM2 (200 kd). Both isoforms are expressed in smooth muscle cells (markers of terminal smooth muscle differentiation)

Staining pattern

  • Cytoplasmic based

References

  1. Wang et al: Antibodies to Novel Myoepithelium - Associated Proteins Distinguish Benign Lesions and Carcinoma in Situ From Invasive Carcinoma of the Breast; Applied Immunohistochemistry; 5(3):141-151, 1997.
  2. Data Sheet, DAKO.
  
88342 - 1st stain
88341 - each additional stain
  
09/06/2017
  
10/17/2018
  
10/02/2020