CD45 by IHC

Alphabetical Test listing

CD45 by IHC-12376 - Technical only, 12379 - Technical & interpretation

CD45 by IHC
12376 - Technical only, 12379 - Technical & interpretation
Leucocyte common antigen

All IHC stains will include a positive control tissue

  • CD45 labels normal and neoplastic B and T lymphocytes; approximately 95% of B cell NHL and 85% of T cell NHL are positive for CD45
  • Myeloid cells (and plasma cells) are weakly positive or negative for LCA
  • Macrophages/histiocytes are usually negative for CD45
  • Non-hematopoietic cells (benign and malignant) are negative for LCA
  • CD45 reactivity may be weak or absent in plasmacytomas and anaplastic large cell lymphomas

Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



  • LCA reacts with a family of 5 glycoproteins present on the surface of the majority of leukocytes

Staining pattern

  • Cell surface membrane (sometimes also in cytoplasm)


  1. Warnke RA et al: Diagnosis of human lymphoma with monoclonal anti-leukocyte antibodies. N Engl J Med 1983:309:1275-81.
  2. Kurtin PJ et al: Leukocyte common antigen - a diagnostic discriminant between hematopoietic and non-hematopoietic neoplasma in paraffin sections using monoclonal antibodies: correlation with immunologic studies and ultrastructural localization. Hum Patho. 1985: 16:353.
88342 - 1st stain
88341 - each additional stain