CD45 by IHC

Alphabetical Test listing

CD45 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
CD45 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Leucocyte common antigen
LCA
  

All IHC stains will include a positive control tissue

  
  • CD45 labels normal and neoplastic B and T lymphocytes; approximately 95% of B cell NHL and 85% of T cell NHL are positive for CD45
  • Myeloid cells (and plasma cells) are weakly positive or negative for LCA
  • Macrophages/histiocytes are usually negative for CD45
  • Non-hematopoietic cells (benign and malignant) are negative for LCA
  • CD45 reactivity may be weak or absent in plasmacytomas and anaplastic large cell lymphomas
  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • LCA reacts with a family of 5 glycoproteins present on the surface of the majority of leukocytes

Staining pattern

  • Cell surface membrane (sometimes also in cytoplasm)

References

  1. Warnke RA et al: Diagnosis of human lymphoma with monoclonal anti-leukocyte antibodies. N Engl J Med 1983:309:1275-81.
  2. Kurtin PJ et al: Leukocyte common antigen - a diagnostic discriminant between hematopoietic and non-hematopoietic neoplasma in paraffin sections using monoclonal antibodies: correlation with immunologic studies and ultrastructural localization. Hum Patho. 1985: 16:353.
  
88342 - 1st stain
88341 - each additional stain
  
10/31/2017
  
10/18/2018
  
10/02/2020