MAFB/IGH FISH
MAFB/IGH FISH-4280G (bone marrow) or 4280H (blood)
LAB4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
Myeloma FISH Panel
Plasma Cell Dyscrasia Panel
Plasma Cell Panel
FISH
Fluorescence In Situ Hybridization
MAFB/IGH
t(14;20)
Rearrangements of MAFB/IGH are recurring abnormalities in plasma cell dyscrasia disorders including Plasma Cell Myeloma. According to current mSMART 3.0 myeloma guidelines, rearrangement of MAFB/IGH is classified as a high-risk cytogenetics category.
This test can be ordered individually or as part of a Plasma Cell Dyscrasia FISH Panel (See the individual panel test for more details).
Bone marrow aspirate:
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
- Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
- Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting
Transfer aspirate from syringe to transport container.
Submit entire specimen unspun. Specimen will be CD138 enriched for plasma cell isolation.
Sodium heparinized whole blood
Touch Prep slides
Bone Marrow Core:
Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline
or
Whole blood:
Dk green Sodium heparin (Na hep), no gel
Whole blood: 5 mL
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting.
Submit entire specimen unspun
AHL Cytogenetics Oncology Request
Ambient
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Delay in transport
- Improper storage
- Leaking container
- Slides broken beyond repair
Fluorescence in situ hybridization (FISH)
Normal or Abnormal with interpretation