PDGFRA (CHIC2) by FISH

Alphabetical Test listing

PDGFRA (CHIC2) by FISH-4280H (blood) or 4280G (bone marrow)

  
PDGFRA (CHIC2) by FISH
  
4280H (blood) or 4280G (bone marrow)
  
4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
4280G - CYTOGENETIC BONE MARROW STUDIES
  
CHIC2
  
CHIC2
Eosinophilia
Eosinophilia FISH Panel
FIP1L1
FIP1L1-PDGFRA fusion
Mast Cell Disease
PDGFRA
4q12
  

Genetic abnormalities that involve the PDGFRA gene cause a type of blood cell cancer called PDGFRA-associated chronic eosinophilic leukemia. The most common of these genetic abnormalities is a deletion of genetic material from chromosome 4 that brings together parts of two genes, FIP1L1 and PDGFRA, creating the FIP1L1-PDGFRA fusion gene. Occasionally, genes other than FIP1L1 are fused with the PDGFRA gene via a translocation between chromosome 4q12 and a partner chromosome. This gene is also involved in systemic mastocytosis and chronic eosinophilic leukemia.  Patients carrying the FIP1L1-PDGFRA fusion show an excellent response to low-dose imatinib mesylate therapy.

  
Sodium heparin (Na hep) whole blood
  
  
5 mL
  
1.0 mL
  
  • Only one specimen tube is required to perform all ordered cytogenetics testing.
    • The more testing ordered, the closer to the optimal volume requirement is preferred.
  • Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting
  

Dk green Sodium heparin (Na hep), no gel

  
Bone marrow
EDTA whole blood (chromosome analysis study is not recommended for this specimen type)
  

EDTA whole blood (not recommended):

Lavender (EDTA), 4mL

  
Bone marrow: 10 mL (minimum 5 mL)
  

Bone marrow

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

  

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel

  

EDTA whole blood:

Lavender (EDTA), 4mL

Bone marrow:

Sterile vial/tube

Dk green Sodium heparin (Na hep), no gel

  
  • AHL Cytogenetics oncology request
  

Ambient

  
Pellets - 2 years
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Leaking container
  
AHL - Cytogenetics: X
  
As needed
  
5 - 7 days
  

Fluorescence in situ hybridization (FISH)

  

Normal or Abnormal with interpretation

  
88271 x 3
88275
  
Yes
  
  
03/16/2021
  
04/06/2021