Factor VIII inhibitor assay

Alphabetical Test listing

Factor VIII inhibitor assay-994

  
Factor VIII inhibitor assay
  
994
  
LAB994
  
MSO
  
Bethesda Titer
Factor 8 inhibitor assay
  
  • Activated partial thromboplastin time (aPTT)
  • aPTT 1:1 mix with normal plasma
  • aPTT 1:1 incubated mix with normal plasma
  • aPTT 1:1 mix with saline
  • Factor VIII activity
  • Factor VIII Bethesda titer
  

Confirmation and characterization of factor VIII inhibitor

  

The patient should not be anticoagulated

  
Sodium citrate (Na cit) plasma
  
  
3 mL
  

Do not draw from an arm with a heparin lock or heparinized catheter

  
  1. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells.
  2. Deliver to a plastic transport tube, cap, and recentifuge for 10 minutes.
  3. Use a second plastic pipette to remove plasma, staying clear of the platelets at the bottom of the tube.
  4. Transfer the plasma into a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as Na cit plasma and freeze immediately.
  

Frozen (strict)

Ambient - 4 hours

  
Esoterix Coagulation Lab (300701) via LabCorp (500350): R-NX
  
Mo - Fr
  
2 - 3 days
  

Clot

  

An interpretive report will be provided

  
85240
85335
85730
85732 x 3
  
Yes
  
  
Result:
500347 APTT 1:1 Normal Pool Mix 5946-9
500349 APTT 1:1 NP Mix, 60 Min,Incub. 30323-0
  
05/29/2019
  
02/17/2021
  
01/08/2024