Alphabetical Test listing



The cryoglobulin test detects antibodies in the blood that may cause sensitivity to low temperatures. Cryoglobulins may be divided into three classes.

  • Type I are monoclonal immunoglobulins, usually IgG or IgM, infrequently IgA, and are usually associated with lymphoproliferative disorders, especially macroglobulinemia or multiple myeloma. These are usually present in high concentrations.
  • Type II cryoglobulins are mixed cryoglobulins consisting of two or more immunoglobulins of different classes, one being a monoclonal protein (with a high proportion being IgM), and the other a polyclonal IgG.
  • Type III cryoglobulins are also mixed cryoglobulins consisting of two or more immunoglobulins of different classes, however, each component is a polyclonal immunoglobulin. These are usually present in low concentrations (<1%), and are associated with various autoimmune diseases, chronic active hepatitis, and other viral infections.

All types of cryoglobulins may be responsible for specific symptoms that occur in response to cold weather, such as Raynaud’s phenomenon, vascular purpura, bleeding tendencies, cold-induced urticaria, and even distal arterial thrombosis with gangrene.


4.0 mL
1.0 mL
Due to the strict temperature requirements of this test, if you do not have a 37 °C incubator or water bath, this test must be collected at an Allina Health Hospital Laboratory reception area.

Not adhering to these strict procedures could result in false negative results. Please instruct your patients accordingly in order to obtain an acceptable specimen.
  1. The specimen must be kept in a 37°C incubator or water bath until clotted.
  2. Spin and separate serum from the cells immediately after centrifugation.
  3. Transfer serum to a False bottom plasma/serum transport vial/tube (AHL)
  4. Maintain at room temperature after separation.

Ambient (strict) - 7 days

Refrigerated - NO

Frozen - NO

  • Samples not clotted in an incubator or water bath at 37°
  • Improper labels (unlabeled or mislabeled)
  • Hemolysis (some procedures)
  • Lipemia
  • Improper anticoagulant or ratio
  • Delay in transport
  • Improper storage temperature affecting results
  • Improper container
  • Leaking container resulting in compromised specimen
  • Quantity not sufficient (QNS)




AHL - Immunology: J
Preliminary - 24 hrs;Final - 7 days