Brucella total antibody confirmation, agglutination, serum

Alphabetical Test listing

Brucella total antibody confirmation, agglutination, serum-994

Test name:

Test number:

994

Excellian order number:

LAB994

Abbreviation:

MSO

Alternate names:

Brucella abortus
Brucella serology
Febrile Agglutinins

Useful for:

Evaluating patients with suspected brucellosis. Test should only be requested if patient has tested positive or equivocal for IgG or IgM by a screening enzyme immunoassay (EIA)

Specimen type:

Serum

Collection container:

Gold serum separator (SST) tube

Volume:

0.5 mL

Minimum volume:

0.2 mL

Collection instructions:

Immediatley following collection, mix sample by gently inverting 5 times

Processing instructions:

Allow sample to clot for a minimum of 30 minutes
Spin within two (2) hours of sample collection

Transport container:

Gold serum separator (SST) tube

Transport and stability:

Refrigerated (preferred) – 14 days

Frozen – 14 days

Ambient - NO

Specimen retention time:

14 days

Reason for rejection:

Gross hemolysis
Gross lipemia
Gross icterus

Performing lab:

Mayo Clinic Laboratories (BRUTA): R - NX

Days set up:

Mo, We

TAT:

7 days

Method:

Agglutination

Reference ranges:

<1:80

Clinical information:

In cases of suspected brucellosis, serology may assist in the diagnosis and play a supplementary role to routine culture. Antibodies to Brucella species may not become detectable until 1 to 2 weeks following the onset of symptoms, so serum specimens drawn during acute disease may be negative by serology in patients with brucellosis. If serology is performed, the Centers for Disease Control and Prevention currently recommends that specimens testing positive or equivocal for IgG or IgM by a screening enzyme immunoassay (EIA) be confirmed by a Brucella-specific agglutination method.

CPT codes:

86622

Date created:

11/23/2021

Review date:

03/08/2023