MDM2 gene amplification differentiates a pathologic diagnosis of well-differentiated liposarcomas from benign lipomas.
Tissue specimens should be placed in a sterile cup with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one (1) hour of the procedure.
Paraffin block or 5 micron cuts mounted on positively charged slides
Fluorescence in situ hybridization (FISH)
Positive (Amplified) result – 25 cells scored with an MDM2:CEP12 ratio ≥2.0
Negative (Non-amplified) result - 25 cells scored with a MDM2:CEP12 ratio <2.0