HMB 45 by IHC

Alphabetical Test listing

HMB 45 by IHC-12376 - Technical only, 12379 - Technical & interpretation

HMB 45 by IHC
12376 - Technical only, 12379 - Technical & interpretation
Human melanoma black
  • All IHC stains will include a positive control tissue
  • HMB45 reacts with most melanomas (approximately 80%) as well as with benign melanocytes such as in junctional melanocytes, Spitz nevi, and cellular blue nevi
  • HMB45 is usually negative in desmoplastic and spindle cell melanomas
  • HMB45 usually is nonreactive with normal melanocytes in normal adult skin, intradermal nevi, and melanophages
  • For melanomas, order both S-100 and HMB45. If lesion is heavily pigmented, consider specifying the alkaline phosphatase (red) staining method
  • No known reactivity with other carcinomas (false reports in literature regarding HMB45 positive breast carcinomas1)
  • Unique reaction of HMB45 in pulmonary lymphangiomyomatosis 2 and angiomyolipomas3

Submit a formalin-fixed, paraffin-embedded tissue


Formalin-fixed, paraffin-embedded (FFPE) tissue block


FFPE tissue section mounted on a charged, unstained slide


Ambient (preferred)

  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
AHL - Immunohistochemistry
Mo - Fr
1 - 2 days

Immunohistochemical staining and microscopic examination


If requested, an interpretive report will be provided



HMB45 recognizes a premelanosome glycoprotein

Staining pattern:

  • Cytoplasmic based (may show focal/mosaic pattern in melanomas)


  1. Bonetti et. al.: False-Positive Immunostaining of Normal Epithelial and Carcinomas with Ascites Fluid Preparations of Antimelanoma Monoclonal Antibody HMB-45; American Journal of Clinical Pathology; 95:454-459, 1991.
  2. Bonetti, et. al.: Transbronchial Biopsy in Lymphangiomyomatosis; American  Journal of Surgical Pathology; 17(11):1092-1102, 1993. Sturtz, et. al.: Angiomyolipomas: The Nature and Expression of the HMB45 Antigen; Modern Pathology; 7(8):842-845, 1994.
88342 - 1st stain
88341 - each additional stain