HMB 45 by IHC

Alphabetical Test listing

HMB 45 by IHC-12376 - Technical only, 12379 - Technical & interpretation

  
HMB 45 by IHC
  
12376 - Technical only, 12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Human melanoma black
  
  • All IHC stains will include a positive control tissue
  
  • HMB45 reacts with most melanomas (approximately 80%) as well as with benign melanocytes such as in junctional melanocytes, Spitz nevi, and cellular blue nevi
  • HMB45 is usually negative in desmoplastic and spindle cell melanomas
  • HMB45 usually is nonreactive with normal melanocytes in normal adult skin, intradermal nevi, and melanophages
  • For melanomas, order both S-100 and HMB45. If lesion is heavily pigmented, consider specifying the alkaline phosphatase (red) staining method
  • No known reactivity with other carcinomas (false reports in literature regarding HMB45 positive breast carcinomas1)
  • Unique reaction of HMB45 in pulmonary lymphangiomyomatosis 2 and angiomyolipomas3
  
Tissue
  

Submit a formalin-fixed, paraffin-embedded tissue

  

Formalin-fixed, paraffin-embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
  • Unlabeled/mislabeled block
  • Insufficient tissue
  • Slides broken beyond repair
  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications:

HMB45 recognizes a premelanosome glycoprotein

Staining pattern:

  • Cytoplasmic based (may show focal/mosaic pattern in melanomas)

References

  1. Bonetti et. al.: False-Positive Immunostaining of Normal Epithelial and Carcinomas with Ascites Fluid Preparations of Antimelanoma Monoclonal Antibody HMB-45; American Journal of Clinical Pathology; 95:454-459, 1991.
  2. Bonetti, et. al.: Transbronchial Biopsy in Lymphangiomyomatosis; American  Journal of Surgical Pathology; 17(11):1092-1102, 1993. Sturtz, et. al.: Angiomyolipomas: The Nature and Expression of the HMB45 Antigen; Modern Pathology; 7(8):842-845, 1994.
  
88342 - 1st stain
88341 - each additional stain
  
07/03/2017
  
10/19/2018
  
10/02/2020