T cell gene rearrangement

Alphabetical Test listing

T cell gene rearrangement-5453

Test name:

Test number:

5453

Excellian order number:

LAB5453

Abbreviation:

Alternate names:

T-cell receptor beta rearrangement (TcRb)
T-cell receptor gamma rearrangement (TcRg)

Useful for:

Detection of clonal rearrangements of T cell receptor genes that are associated with malignant processes

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL,

Volume:

4.0 mL

Minimum volume:

2.0 mL

Collection instructions:

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

Transport container:

Lavender (EDTA), 4mL

Alternate specimen type:

ACD whole blood
Sodium citrate (Na cit) whole blood
Bone marrow
Tissue

Alternate collection container:

Yellow ACD (A or B)

Lt blue Sodium citrate (Na Cit) - 2.7mL

Bone Marrow

Lavender (EDTA), 4mL

Fresh tissue

RPMI with Hepes/Pen strep/FBS -15 mL

Alternate specimen volume:

Whole blood 4.0mL (minimum 1.0 mL)
Bone marrow 5.0cc (minimum 1.0cc)
Tissue 200mg

Alternate processing instructions:

Bone marrow

Submit entire specimen

Tissue

Frozen preferred if not tested immediately
Freeze as quickly as possible at -20 to -70°C
Fresh tissues may be submitted in RPMI
Best results when used within 3 days
Recommended for tissues with high probability of immediate or pending orders
Formalin fixed tissue in a paraffin block (B3 and B5 fixatives are not recommended)

Questions call (612) 863-4475

Alternate transport container:

Yellow ACD (A or B)

Lt blue Sodium citrate (NaCit)

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

Formalin-fixed, paraffin embedded (FFPE) tissue block

Required forms/information:

Molecular Medicare billing request

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medicare billing request form to notify us of the need for Allina Health Laboratory to bill insurance.

Transport and stability:

Whole blood or bone marrow:

Refrigerated (preferred)

Ambient (OK)

Tissue:

Ambient

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Improper blood/anticoagulant ratio
Delay in transport
Improper storage
Inappropriate timing of collection
Interfering substances
Leaking container

Performing lab:

AHL - Molecular Diagnostics: D

Days set up:

Varies

TAT:

8 days

Method:

PCR and capillary electrophoresis

Preauthorization:

This test may require preauthorization from the insurance provider. Check the payer guidelines and, if needed, obtain the pre-authorization prior to sample collection.

CPT codes:

81340
81342

Subject to medical necessity (Y/N):

Yes

Other billing information:

Medical necessity

Molecular Medicare billing request

Date created:

06/22/2006

Revised date:

05/15/2024

Review date:

01/15/2024