16s rDNA Broad Range Bacterial PCR

Alphabetical Test listing

16s rDNA Broad Range Bacterial PCR-12804

  
16s rDNA Broad Range Bacterial PCR
  
12804
  
LAB12804
  
16S
  
16s Gene sequencing
16 s
16-s
16s sequencying
16s RDNA sequencing
AFB sequencing
Bacterial sequencing
Broad spectrum bacterial PCR
FNA
Syphilis PCR
Treponema PCR
NextGen Sequencing
Next Generation Sequencing
  
Fresh frozen tissue (bone, bone marrow, fine needle aspirate, lung, lymph, muscle or spleen)
  

Sterile vial/container

  
1 cm x 1 cm
  
None
Senstivity is decreased with lower volumes of tissue or fluid
  

Preferred - Fluid or tissue for broad range bacterial DNA testing should be put in a sterile container by the provider at the time of the procedure.  This ensures that the specimen won’t be opened until it reaches the testing laboratory and prevents the opportunity for contamination.

Acceptable - OK to add on to an open container.  Order must be placed within 7 days of collection.  Freeze immediately upon receipt of 16s order.

28s, 26s, or 23s (broad range fungal PCR) may be ordered on this same specimen, and will be performed at an additional charge.  Order as an MSO.

Contact the laboratory medical director through Client Services if there are questions.

  

Sterile vial/container

  
Aspirate/fluid, undiluted (abdominal, abcess, amniotic, ascitic, BAL, Bile, Cerebral spinal fluid, cyst, paracentesis, pericardial, pleural, synovial thoracentesis or vitreous biopsy
  
2.0 mL
There is no minimum volume, however, sensitivity is decreased with lower volumes
  

For all sites other than Phillips Eye:

For Phillips Eye (PEA) only:

  • U of Washington request form
    • The following items are required:
      • Patient name
      • Patient DOB
      • Ordering physician
      • Ordering physician phone number
      • Specimen source
      • Date and time collected
      • ICD/Diagnosis

 

  

Frozen (preferred) - 21 days

Refrigerated - 7 days

Ambient (room temp) - 2 hours

  
  • Diluted fluid sample, including eye/vitreous 
  • Specimens collected on swabs
  
Hennepin Health/HCMC (LAB0001738): R-HM
  
3-7 days
  

PCR Sequencing

  

No bacterial 16S rDNA detected

  
87801
87798 will be added if results are positive
  
09/27/2011
  
08/06/2024
  
08/06/2024