20q deletion by FISH

Alphabetical Test listing

20q deletion by FISH-4280G (bone marrow) or 4280H (blood)

Test name:

Test number:

4280G (bone marrow) or 4280H (blood)

Excellian order number:

4280G - CYTOGENETIC BONE MARROW STUDIES
4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES

Abbreviation:

20Q-

Alternate names:

Deletion 20q
FISH
Fluorescence In Situ Hybridization
MDS
Myelodysplastic Syndrome
MDS
Myelodysplastic Syndrome
Myeloproliferative Neoplasm
MPN

Useful for:

Deletion 20q is a recurring abnormality in myelodysplastic (MDS) syndromes. Of note, a 20q- in the absence of morphologic criteria is not considered definitive evidence of MDS. According to current NCCN MDS guidelines, a 20q- result is classified as a good cytogenetics risk factor.

This test can be ordered individually or as part of the MDS FISH Panel. See the panel test for more details.

Specimen type:

Bone Marrow aspirate

Collection container:

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep ), no gel
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times, to prevent clotting

Volume:

5 mL

Minimum volume:

3 mL

Collection instructions:

Transfer aspirate from syringe to transport container.

Processing instructions:

Submit entire specimen unspun.

Transport container:

Sterile vial/tube

Dk green Sodium heparin (Na hep ), no gel

Alternate specimen type:

Bone Marrow Core
Whole blood
Touch Prep Slides

Alternate collection container:

Bone Marrow Core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

Whole blood:

Dk green Sodium heparin (Na hep ), no gel

Alternate specimen volume:

Bone Marrow core: 10 mm
Whole blood: 5 mL

Alternate collection instructions:

Immediatley following collection, mix sample by inverting 8 - 10 times to prevent clotting

Alternate processing instructions:

Submit entire specimen unspun

Alternate transport container:

Sterile tube/cup

Dk green Sodium heparin (Na hep l ), no gel

Required forms/information:

AHL Cytogenetics Oncology Request

Transport and stability:

Ambient

Specimen retention time:

Specimens retained until completion of all testing

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Delay in transport
Improper storage
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Cytogenetics: X

Days set up:

Mo - Fr

TAT:

7 business days

Method:

Fluorescence in situ hybridization (FISH)

Reference ranges:

Normal or Abnormal with interpretation.

CPT codes:

88271 x 2
88275
88291

Subject to medical necessity (Y/N):

Yes

LCD/NCD:

Medical necessity

Date created:

03/26/2021

Revised date:

09/28/2022