Plasma cell monitoring

Alphabetical Test listing

Plasma cell monitoring-12420

  
Plasma cell monitoring
  
12420
  
LAB12420
  
PATR
  
Flow
Flow cytometry
Immunophenotyping
MGUS
MM
Monoclonal gammopathy, plasma cell
Multiple Myeloma
Multiple Myeloma monitoring
P2
Plasma cell Immunophenotyping
Plasma cell follow-up
  
  • CD19
  • CD20
  • CD38
  • CD45
  • CD56
  • IRF4 (nuclear)
  • Kappa (cytoplasmic)
  • Lambda(cytoplasmic)
  • VS38(cytoplasmic)
  

Detecting residual disease in treated multiple myeloma patients at follow-up

  

Pathology orders only

The P2 assay is a high-sensitivity assay validated to a sensitivity of 0.01% (1 abnormal plasma cell/10,000 normal cells). This assay is NOT a minimal/measureable residual (MRD) assay, which requires a validated sensitivity of 0.001% (1/100,000). For follow-up of patients who are negative for residual abnormal plasma cells in the P2 assay, we recommend EDTA-anticoagulated bone marrow aspirate be submitted to the Mayo Clinic Laboratories for formal MRD testing (Mayo Clinic Laboratories test code: MRDMM).

  
Bone marrow
  

Heparinized syringe

  
3.0 mL
  
1.0 mL
  

Collect 3.0 mL (minimum 1.0mL) bone marrow aspirate in a sodium heparinized syringe and transfer to an anticoagulant free tube.

Bone marrow specimen transport kit

  
Bone marrow core
  
  

 

  

Bone marrow aspirate:

If you do not use a heparinized syringe to collect the sample:

Dk green Sodium heparin (Na hep), no gel (preferred)

Lavender (EDTA), 4mL (acceptable)

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting

Bone marrow core:

RPMI with Hepes/Pen strep/FBS -15 mL

  

Ambient (preferred) - 72 hours

Refrigerated (discouraged)

Frozen - NO

  
14 days
  
  • Improper labels (unlabeled or mislabeled)
  • Improper anticoagulant or ratio
  • Delay in transport
  • Improper storage temperature affecting results
  • Improper container
  • Leaking container resulting in compromised specimen
  • Quantity not sufficient (QNS)
  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow cytometry

  

Tubes:

P2

Markers:

CD19, CD20, CD38, CD45, CD56, IRF4 (nuclear), Kappa (cytoplasmic), Lambda (cytoplasmic) and VS38 (cytoplasmic).

  
The P2 assay is a high-sensitivity assay validated to a sensitivity of 0.01% (1 abnormal plasma cell/10,000 normal cells). This assay is NOT a minimal/measureable residual (MRD) assay, which requires a validated sensitivity of 0.001% (1/100,000). For follow-up of patients who are negative for residual abnormal plasma cells in the P2 assay, we recommend EDTA-anticoagulated bone marrow aspirate be submitted to the Mayo Clinic Laboratories for formal MRD testing (MCL test code: MRDMM).
  
88184
88185 × 8*
*Charges may vary based on immunophenotype of cells in the specimen
  
88184 - 17021303
88185 (each) - 17021304
  
88184 - 30881840
88185 (each) - 308818500
  
06/03/2021
  
01/09/2024