Serotonin release assay (SRA)
Serotonin release assay (SRA)-994
Heparin-dependent platelet antibody
SRA, High Dose Heparin
SRA, Interpretation
Confirmatory assay used in the evaluation of heparin-induced thrombocytopenia (HIT).
Submission of the minimum volume does not allow for repeat testing
Immediately following collection, thoroughly mix sample by gently inverting 5 times
- Allow sample to clot for a minimum of 30 minutes
- Spin within two (2) hours of sample collection
- Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum
- Freeze
- Allow sample to clot
- Spin
- Transfer serum to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp), labelled as serum, within two (2) hours of sample collection
- Freeze
Frozen
Room temperature specimen received; grossly hemolyzed, icteric, and lipemic samples
The SRA employs washed donor platelets and detects their activation by measuring the release of endogenous serotonin induced by addition of patient serum. The serotonin levels are measured by a highly sensitive liquid chromatography/ tandem mass spectroscopy method. Therapeutic levels of heparin included in the assay mix allow for the formation of PF4/heparin complexes on the donor platelet surface to provide an antigen target for the HIT antibodies. The specificity of the assay is increased by assessing the inhibition of platelet activation by adding heparin in excess, thus proving the heparin dependency of the serotonin release.
An interpretive report will be provided
SRA High Dose Heparin 50727-7