Specimen type:  

Tumor tissue (fresh)

Collection container:  

Sterile culture medium or a sterile cup with saline

Volume:  

5 mm (3)

Transport container:  

Sterile culture medium or in a sterile cup with sterile saline

Alternate specimen type:  

Tumor tissue FFPE block or slides

Alternate specimen volume:  

Block
Slides: 2 plus one H & E stained slide

Alternate processing instructions:  

Fix the tissue in 10% formalin and prepare a Formalin-fixed, paraffin-embedded (FFPE) tissue block

Either the entire block, or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing

Alternate transport container:  

FFPE tissue block or

Tissue section mounted on charged, unstained slides

Required forms/information:  

AHL Cytogenetics oncology request

Transport and stability:  

Ambient

Specimen retention time:  

Paraffin specimens:
Slides - 6 months
Blocks will be returned post reporting

Fresh tissue – until testing is reported

Reason for rejection:  

• Improper label (unlabeled or mislabeled)
• Wrong container (anticoagulant or solution)
• Delay in transport
• Improper storage
• Leaking container
• Slides broken beyond repair

Performing lab:  

AHL - Cytogenetics: X

TAT:  

5 days

Method:  

Fluorescence in situ hybridization (FISH)

Reference ranges:  

Normal or Abnormal with interpretation

CPT codes:  

Paraffin specimens:

For MYC negative results, 88366 will be charged.
If MYC is positive for rearrangement, BCL2/IGH and BCL6 will be reflexed and 88366 x 3 – Qualitative Morphometric FISH codes will be charged.
If no cells are available for analysis, no analysis charges will be incurred

Fresh tissue specimens:
For MYC negative results, quantitative 88271 x 2 and 88275 x 1 codes will be charged.
If MYC is positive for rearrangement, BCL2/IGH and BCL6 will be reflexed and 88271 x 6 and 88275 x 3 codes will be charged.
If no cells are available for analysis, no analysis charges will be incurred

Subject to medical necessity (Y/N):  

Yes

LCD/NCD:  

Medical necessity

Date created:  

03/26/2021