Hairy cell leukemia, diagnostic

Alphabetical Test listing

Hairy cell leukemia, diagnostic-12420

  
Hairy cell leukemia, diagnostic
  
12420
  
LAB12420
  
HC
  
Flow
Flow Cytometry
Hairy cell
Immunophenotyping
Leukemia/Lymphoma panel
  
Tubes:
B1
B2
B3

Markers:
CD2
CD3
CD5
CD10
CD11c
CD19
CD20
CD22
CD23
CD25
CD38
CD43
CD45
CD79b
CD103
CD123
CD200
Kappa
Lambda

If the B cells are negative or equivocal for surface Kappa/Lambda, testing will reflex to an additional tube at an additional charge
  

B Cell Leukemia /Lymphoma panel plus hairy cell-associated markers

  

Pathology orders only

  
EDTA whole blood
  
  
4 mL
  
1.0 mL
  

Immediately following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

  

Lavender (EDTA), 4mL

  
Body fluid
Bone marrow
Spinal fluid (CSF)
Tissue
  
Varies; see Alternate collection instructions
  

Body fluid

  • Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
    • Do not add anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Include representative slides (will be returned)

Bone marrow

  • Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
    • Specimens collected in EDTA are acceptable, but not preferred
  • Transfer to an anticoagulant free sterile tube
  • If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable
  • Submit representative slides (will be returned)

Cerebrospinal fluid (CSF)

  • Collect 5 mL (minimum 1 mL) in a sterile vial/container.
    • Do NOT add any anticoagulant or holding media
    • The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
  • Send representative slides (will be returned)

Tissue

  • Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
  • Send representative slides (will be returned)
  

Body fluid

Falcon vial/tube - 15mL

Bone marrow

Bone marrow transport vial/tube

Cerebrospinal fluid (CSF)

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

  

Include previous Allina case number, if applicable.

  

EDTA whole blood

Ambient

Body fluid

Refrigerated

Bone marrow

Ambient

Spinal fluid

Refrigerated

Tissue

Refrigerated

  
14 days
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
    • Tissues submitted in saline or B+ fixative are not acceptable and will be rejected
  • Improper blood/anticoagulant ratio
  • Delay in transport
  • Improper storage
  • Inappropriate timing of collection
  • Interfering substances
  • Leaking container
  
AHL - Flow Cytometry: F
  
Mo - Fr
  
2 days
  

Flow cytometry

  

Tubes and Markers:

Tubes: B1, B2, and B3 

Markers: CD2, CD3, CD5, CD10, CD11c, CD19, CD20, CD22, CD23, CD25, CD38, CD43, CD45, CD79b, CD103, CD123, CD200, Kappa and Lambda

  
88184
88185 x 18

If indicated:
88185 x 2
  
08/04/2021
  
02/13/2024
  
01/09/2024