CD57 by IHC

Alphabetical Test listing

CD57 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

CD57 by IHC

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Alternate names:

Leu-7

Tests included:

All IHC stains will include a positive control tissue

Useful for:

CD57 stains the small lymphocytes in nodular lymphocyte predominance Hodgkin's disease (NLPHD), in a ring-like pattern surrounding L&H cells (this pattern can distinguish NLPHD from nodular sclerosing Hodgkin's disease, T-cell-rich B-cell lymphoma, and follicular NHL
CD57 is useful in tumor diagnosis as a specific but relatively insensitive pan-neuroendocrine marker, usually showing positive staining in neuroblastomas, retinoblastomas, PNETs, neuroepitheliomas, carcinoids, Merkel cell tumors of the skin, paragangliomas, pheochromocytomas, small cell neuroendocrine carcinomas of the lung, urinary bladder, colon, rectum, and endometrium, medullary carcinomas of the thyroid, and some pancreatic endocrine tumors
Occasional cases of Ewing's sarcoma, granular cell myoblastoma, Wilms' tumor, leiomyosarcoma, synovial sarcoma, epithelioid sarcoma, clear cell sarcoma, embryonal rhabdomyosarcomas, and malignant melanomas also stain positively for CD57
Positive staining is also seen in other neural tumors such as schwannomas, neurofibromas, neurofibrosarcomas, astrocytomas and ependymomas
A high percentage of oligodendrogliomas show positive cell surface membrane staining for CD57
Positive staining for CD57 is seen in normal and hyperplastic prostate and prostatic adenocarcinomas
CD57 is absent in squamous cell carcinomas and adenocarcinomas (other thanreports of rare prostate tumors as above)

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin-embedded tissue

Transport container:

Formalin-fixed, paraffin-embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

Anti CD57 recognizes a 110 KD glycoprotein human lymphocyte antigen that is present on approx. 25% of adult peripheral blood mononuclear cells
CD57 is expressed on a subset of NK cells and a subset of germinal center T-cells
This antibody also recognizes a carbohydrate epitope on sub-populations of several cell adhesion molecules including N-CAM and myelin-associated glycoprotein (MAG)
In normal tissues, CD57 stains a common neuro-endodermal antigen with positivity in cells of the CNS, the adrenal medulla, islets of Langerhans and the cells of the APUD system in the lung and gut

Staining pattern

Cytoplasmic and cell surface membrane based staining

References

Kamel OW, et al: Leu 7 (CD57) reactivity distinguishes nodular lymphocyte predominance Hodgkin's disease from nodular sclerosing Hodgkin's disease, T-cell- rich B-cell lymphoma and follicular lymphoma. American Journal of Pathology, Vol 142, 541-546, 1993.
Arber DA, Weiss LM: CD57: a review. Appl Immuno 1995, 3:137-152.
Michels S et al: Leu-7 in small cell neoplasms. An immunohistochemical study with ultrastructural correlations. Cancer 1987, 60:2958-2964.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

06/16/2017

Revised date:

10/18/2018

Review date:

01/12/2024