AML panel by FISH
AML panel by FISH-Order individual components; 4280H (blood) or 4280G (bone marrow)
LAB4280G - CYTOGENETIC BONE MARROW STUDIES
AML
CBFB-MYH11
Deletion 5q31
FISH
Fluorescence in situ hybridization
inv(16)
Monosomy 5
Monosomy 7
RUNX/RUNX1
t(16;16)
t(8;21)
5q-
-5
-7
- CBFB
- MLL
- RUNX/RUNX1
- 5Q-
- 7Q-
The AML FISH panel is useful for detecting the prognostically significant cytogenetic FISH abnormalities used in Acute Myeloid Leukemia according to the NCCN guidelines.
- Only one specimen tube is required to perform all ordered cytogenetics testing
- The more testing requested, the closer to the optimal volume requirements is required
- Immediately following collection, mix sample by inverting 5 - 10 times to prevent clotting
Dk green Sodium heparin (Na hep), no gel
EDTA whole blood (a chromosome analysis study is not recommended for this specimen type)
Bone marrow
Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube
- or -
Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel
EDTA whole blood:
Lavender (EDTA), 4mL
Bone marrow:
Sterile vial/tube
Dk green Sodium heparin (Na hep), no gel
- AHL Cytogenetics oncology request
Ambient
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Improper blood/anticoagulant ratio
- Delay in transport
- Improper storage
- Leaking container
Fluorescence in situ hybridization (FISH)
Normal or Abnormal with interpretation.
Cytogenetic favorable prognostic indicators include: t(8;21) and inv(16) or t(16;16).
Cytogenetic intermediate prognostic indicators include: t(9;11) and abnormalities not classified as favorable or unfavorable.
Cytogenetic unfavorable prognostic indicators include: variant t(?;11) MLL rearrangements, -5 and 5q-, -7, and 17p- or TP53 deletions.