Treponema pallidum by IHC

Alphabetical Test listing

Treponema pallidum by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Alternate names:

Spirochete

Tests included:

All IHC stains will include a positive control tissue

Useful for:

T Pallidum is used to identify syphilis in tissue based material
This stain is an important adjunct in the identification of syphilis, since the organism is refractory to culture, and histochemical stains are difficult to interpret

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin embedded tissue block

Transport container:

Formalin-fixed, paraffin embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

T Pallidum identifies the causative agent of syphilis

Staining pattern

Red, corkscrew bacterial pattern of staining (a red chromogen is used with this antibody)

References

Guarner J et al: Congenital syphilis in a newborn: an immunopathologic study. Mod Pathol 1999; 12:82-87.
Beckett JH et al: Immunoperoxidase localization of treponema pallidum: its use in formaldehyde-fixed and paraffin-embedded tissue sections. Arch Pathol Lab med 1979 Mar; 103(3): 135-8.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

09/13/2017

Revised date:

10/17/2018

Review date:

01/12/2024